NSJ Bioreagents

STAT3 Antibody (pTyr705)

Product Code:
 
NSJ-R20279
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
RM261
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store the recombinant phospho-Stat3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
1 / 2
Western blot of A431 cell lysate, untreated (-) or treated (+) with EGF, using recombinant phospho-Stat3 antibody at 1:1000.~
2 / 2
IHC testing of FFPE human breast cancer tissue with recombinant phospho-Stat3 antibody at 1:10,000.
❮❯

Western blot of A431 cell lysate, untreated (-) or treated (+) with EGF, using recombinant phospho-Stat3 antibody at 1:1000.~
IHC testing of FFPE human breast cancer tissue with recombinant phospho-Stat3 antibody at 1:10,000.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-R20279-0.1ML100ul£524.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details:
Immunohistochemistry (FFPE): 1:1000-1:10;000 (1),Western blot: 1:1000-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant phospho-Stat3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Description:
This recombinant phospho-Stat3 antibody reacts to human Stat3 only when phosphorylated at Tyr705. There is no cross-reactivity to Stat3 without phosphorylation at Tyr705. It may also react to mouse or rat phospho-Stat3 (Tyr705), as predicted by immunogen homology.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A phospho-peptide corresponding to human phospho-Stat3 (Tyr705) was used as the immunogen for this recombinant phospho-Stat3 antibody.
Limitation:
This recombinant phospho-Stat3 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P40763

Documents