NSJ Bioreagents

HER2 Antibody / ErbB2

Product Code:
 
NSJ-R20258
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
RM228
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store the recombinant HER2 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
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Western blot of MCF7, PC3, Jurkat, and A375 cell lysate using recombinant HER2 antibody. Only the breast cancer line MCF7 showed a response.~
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IHC testing of FFPE human breast cancer tissue with recombinant HER2 antibody. Expected molec
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Western blot of MCF7, PC3, Jurkat, and A375 cell lysate using recombinant HER2 antibody. Only the breast cancer line MCF7 showed a response.~
IHC testing of FFPE human breast cancer tissue with recombinant HER2 antibody. Expected molec

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NSJ-R20258-0.1ML0.1ml£524.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1:100 - 1:400 (1),Western blot: 1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant HER2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Description:
This recombinant HER2 antibody reacts to Human c-erbB-2/HER-2. It may also react to mouse or rat c-erbB-2/HER-2, as predicted by immunogen homology.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to the C-terminus of human c-erbB2/HER2 was used as the immunogen for this recombinant HER2 antibody.
Limitation:
This recombinant HER2 antibody is available for research use only.
Localization:
Cell surface, cytoplasm, nucleus
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P04626

Documents