NSJ Bioreagents

EpCAM Antibody

Product Code:
 
NSJ-V2691CF488
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
EGP40/826
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
 
Store the EpCAM antibody at 2-8oC protected from light.
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Immunofluorescent staining of human SK-OV-3 cells with CF488-conjugated EpCAM antibody (clone EGP40/826, green), phalloidin (red) and DAPI nuclear stain (blue).~
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Immunofluorescent staining of human SK-OV-3 cells with isotype cont
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Immunofluorescent staining of human SK-OV-3 cells with CF488-conjugated EpCAM antibody (clone EGP40/826, green), phalloidin (red) and DAPI nuclear stain (blue).~
Immunofluorescent staining of human SK-OV-3 cells with isotype cont

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NSJ-V2691CF488-100T100 Tests£534.00
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This product comes from: United States.
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Further Information

Application Details:
Flow cytometry: 5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood,Immunofluorescence: 1:50-1:100
Application Note:
Optimal dilution of the EpCAM antibody should be determined by the researcher.
Description:
Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.
Format:
CF488 Conjugate
Formulation:
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A synthetic peptide (around aa 20-60) from the N-terminus of the human protein was used as the immunogen for the EpCAM antibody.
Limitation:
This EpCAM antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography
Uniprot #:
P16422

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