NSJ Bioreagents

Desmin Antibody (Muscle cell marker)

Product Code:
 
NSJ-V3195SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
DES/1711
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the Desmin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC testing of FFPE human uterus with Desmin antibody (clone DES/1711). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.~
2 / 2
SDS-PAGE Analysis of Purified, BSA-Free Desmin Antibody (clone DES/1711). Confirmat
❮❯

IHC testing of FFPE human uterus with Desmin antibody (clone DES/1711). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.~
SDS-PAGE Analysis of Purified, BSA-Free Desmin Antibody (clone DES/1711). Confirmat

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NSJ-V3195SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunofluorescence: 1-2ug/ml,Flow cytometry: 0.5-1ug/million cells,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Desmin antibody should be determined by the researcher.
Description:
Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain a-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles. Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human Desmin was used as the immunogen for the Desmin antibody.
Limitation:
This Desmin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P17661

Documents