NSJ Bioreagents

CD74 / CLIP Antibody

Product Code:
 
NSJ-V8263SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
CLIP/3127R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant CD74 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 4
Western blot testing of human 293T cell lysate with recombinant CD74 antibody (clone CLIP/3127R). Expected molecular weight: 33-43 kDa.
2 / 4
Flow cytometry testing of human Raji cells with recombinant CD74 antibody (clone CLIP/3127R); Red=isotype control, Blue= recombinant CD74 antibody.
3 / 4
IHC staining of FFPE human tonsil with recombinant CD74 antibody (clone CLIP/3127R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 4
SDS-PAGE analysis of purified, BSA-free recombinant CD74 antibody (clone CLIP/3127R) as confirmation of integrity and purity.
❮❯

Western blot testing of human 293T cell lysate with recombinant CD74 antibody (clone CLIP/3127R). Expected molecular weight: 33-43 kDa.
Flow cytometry testing of human Raji cells with recombinant CD74 antibody (clone CLIP/3127R); Red=isotype control, Blue= recombinant CD74 antibody.
IHC staining of FFPE human tonsil with recombinant CD74 antibody (clone CLIP/3127R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CD74 antibody (clone CLIP/3127R) as confirmation of integrity and purity.

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NSJ-V8263SAF-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 1-2ug/million cells in 0.1ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
SU-DHL-4 lymphoma cells were used as the immunogen for this recombinant CD74 antibody.
Limitation:
This recombinant CD74 antibody is available for research use only.
Localization:
Cell surface and paranuclear globular
Purity:
Protein A affinity chromatography
Uniprot #:
P04233

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