NSJ Bioreagents

CD99 Antibody

Product Code:
 
NSJ-V3749SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
CDLA99-2R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the recombinant CD99 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC testing of FFPE human Ewing's sarcoma with recombinant CD99 antibody (clone CDLA99-2R). Required HIER: steam sections in 10mM Citrate buffer, pH 6.0, for 10-20 min.
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IHC testing of FFPE human Ewing's sarcoma with recombinant CD99 antibody (clone CDLA99-2R). Required HIER: steam sections in 10mM Citrate buffer, pH 6.0, for 10-20 min.

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NSJ-V3749SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 5-10ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant CD99 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
CD99 is found on the cell surface of Ewing's sarcoma tumors and is positive in granulosa cell tumors. It is more expressed in malignant gliomas than in the brain, and such overexpression results in higher levels of invasiveness and lower rates of survival. Antibodies to CD99 are used in diagnostic immunohistochemistry to distinguish Ewing's sarcoma from other tumours of similar histological appearance, as well as for the identification of thymic tumours, and of spindle cell tumours, such as synovial sarcoma, haemangiopericytoma, and meningioma.[Wiki]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Human protein was used as the immunogen for the recombinant CD99 antibody.
Limitation:
This recombinant CD99 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein A affinity chromatography
Uniprot #:
P14209

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