NSJ Bioreagents

DC-SIGN Antibody / CD209

Product Code:
 
NSJ-V3452SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
C209/1781
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the DC-SIGN antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
IHC testing of FFPE human lung carcinoma with DC-SIGN antibody (clone C209/1781). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20.
2 / 3
IHC testing of FFPE human small intestine with DC-SIGN antibody (clone C209/1781). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20.
3 / 3
Western blot testing of human liver and spleen lysate with DC-SIGN antibody. Predicted molecular weight ~46 kDa.
❮❯

IHC testing of FFPE human lung carcinoma with DC-SIGN antibody (clone C209/1781). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20.
IHC testing of FFPE human small intestine with DC-SIGN antibody (clone C209/1781). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20.
Western blot testing of human liver and spleen lysate with DC-SIGN antibody. Predicted molecular weight ~46 kDa.

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NSJ-V3452SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
Titering of the DC-SIGN antibody may be required for optimal performance.
Description:
DC-SIGN / CD209 is a transmembrane receptor that is expressed on the surface of dendritic cells and macrophages. It is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization, which allows the receptor to bind multivalent ligands with high avidity.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A human partial recombinant protein was used as the immunogen for the DC-SIGN antibody.
Limitation:
This DC-SIGN antibody is available for research use only.
Localization:
Cytoplasmic, membranous, secreted
Purity:
Protein G affinity chromatography
Uniprot #:
Q9NNX6

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