NSJ Bioreagents

Desmoglein 3 Antibody / DSG3

Product Code:
 
NSJ-V3246SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
5G11
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the Desmoglein 3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC testing of FFPE human skin with Desmoglein 3 antibody (clone 5G11). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.~
2 / 2
SDS-PAGE Analysis of Purified, BSA-Free Desmoglein 3 Antibody (clone 5G11). Confirm
❮❯

IHC testing of FFPE human skin with Desmoglein 3 antibody (clone 5G11). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.~
SDS-PAGE Analysis of Purified, BSA-Free Desmoglein 3 Antibody (clone 5G11). Confirm

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NSJ-V3246SAF-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Desmoglein 3 antibody should be determined by the researcher.
Description:
Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members are identified and all are members of the cadherin cell adhesion molecule superfamily.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant human protein corresponding to the extracellular portion of DSG3 was used as the immunogen for the Desmoglein 3 antibody.
Limitation:
This Desmoglein 3 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography
Uniprot #:
P32926

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