NSJ Bioreagents

CDH17 Antibody / Cadherin 17 (Antigen affinity purified)

Product Code:
 
NSJ-RQ6713
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
After reconstitution the CDH17 antibody can be stored for up to one month at 4°C. For long-term aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 7
IHC staining of FFPE mouse colon tissue with CDH17 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 7
IHC staining of FFPE rat colon tissue with CDH17 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 7
IHC staining of FFPE human rectal cancer tissue with CDH17 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
Immunofluorescent staining of FFPE human intestinal cancer tissue with CDH17 antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 7
Immunofluorescent staining of FFPE human intestinal cancer tissue with CDH17 antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 7
Immunofluorescent staining of FFPE human Caco-2 cells with CDH17 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
7 / 7
Flow cytometry testing of fixed and permeabilized human Caco-2 cells with CDH17 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDH17 antibody.
❮❯

IHC staining of FFPE mouse colon tissue with CDH17 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat colon tissue with CDH17 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer tissue with CDH17 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human intestinal cancer tissue with CDH17 antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human intestinal cancer tissue with CDH17 antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human Caco-2 cells with CDH17 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of fixed and permeabilized human Caco-2 cells with CDH17 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDH17 antibody.

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CodeSizePrice
NSJ-RQ6713-100ug100ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CDH17 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Cadherin-17 is a protein that in humans is encoded by the CDH17 gene. By somatic cell hybrid analysis and fluorescence in situ hybridization, CDH17 gene is mapped to 8q22.1. This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids E24-Q695) was used as the immunogen for the CDH17 antibody.
Limitation:
This CDH17 antibody is available for research use only.
Localization:
Cell surface, Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q12864

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