NSJ Bioreagents

MIF Antibody / Macrophage migration inhibitory factor

Product Code:
 
NSJ-V4856SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
MIF/6282
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the MIF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 2
Western blot testing of human HEK293 cell lysate with MIF antibody (clone MIF/6282). Predicted molecular weight ~13 kDa.~
2 / 2
IHC staining of FFPE human prostate tissue with MIF antibody (clone MIF/6282). HIER: boil tissue sections in pH 9 10m

Western blot testing of human HEK293 cell lysate with MIF antibody (clone MIF/6282). Predicted molecular weight ~13 kDa.~
IHC staining of FFPE human prostate tissue with MIF antibody (clone MIF/6282). HIER: boil tissue sections in pH 9 10m

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NSJ-V4856SAF-100UG100 ug£534.00
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Description:
Macrophage migration inhibitory factor, known as MIF or Glycosylation inhibiting factor, is a secreted, homotrimeric, pro-inflammatory cytokine that modulates macrophage and T cell function and is an important regulator of host response to infection. MIF is expressed at sites of inflammation, which suggests that it plays a role in regulating macrophage function in host defense. MIF is produced by the pituitary gland and is found in monocytes, macrophages, differentiating immunological cells in the eye lens and brain, and fibroblasts. Elevated levels of MIF protein are detected in the plasma of patients with severe sepsis or septic shock, a condition where MIF influences endotoxic shock by enhancing the production of other inflammatory cytokines including tumor necrosis factor Alpha (TNF Alpha), interleukin-1 (IL-1) and interferon-Gamma (IFN-Gamma). MIF promotes the systemic inflammatory response by counter-regulating glucocorticoid-mediated inhibition of immune-cell activation and proinflammatory cytokine production. MIF may mediate tissue destruction through the induction of proteinases.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant fragment of human MIF protein was used as the immunogen for the MIF antibody.
Limitation:
This MIF antibody is available for research use only.
Localization:
Secreted, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P14174

Documents