NSJ Bioreagents

BAG-1 Antibody / BAG-1L p50 specific

Product Code:
 
NSJ-R20344
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
RM310
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant BAG-1 antibody at -20°C.
1 / 4
Western blot testing of human HeLa cell lysate with recombinant BAG-1 antibody at 1:1000. Expected molecular weight of the BAG-1L isoform: ~50 kDa.
2 / 4
ICC staining of BAG-1L overexpressing FFPE LNCaP cells with recombinant BAG-1 antibody at 1:2000.
3 / 4
ICC staining of FFPE human 22RV1 cells with recombinant BAG-1 antibody at 1:2000.
4 / 4
ICC staining of FFPE human 22RV1 cells with recombinant BAG-1 antibody at 1:2000.
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Western blot testing of human HeLa cell lysate with recombinant BAG-1 antibody at 1:1000. Expected molecular weight of the BAG-1L isoform: ~50 kDa.
ICC staining of BAG-1L overexpressing FFPE LNCaP cells with recombinant BAG-1 antibody at 1:2000.
ICC staining of FFPE human 22RV1 cells with recombinant BAG-1 antibody at 1:2000.
ICC staining of FFPE human 22RV1 cells with recombinant BAG-1 antibody at 1:2000.

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NSJ-R20344-0.1ML100 ul£558.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1:500-1:2000,Western blot: 1:1000-1:2000,Immunocytochemistry: 1:200-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant BAG-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to the BAG-1L (p50) isoform of human BAG-1. It does not cross react to BAG-1M or BAG-1S.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to residues near the N-terminus of the human BAG-1L isoform was used as the immunogen for the BAG-1 antibody.
Limitation:
This recombinant BAG-1L antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
Q99933

Documents