NSJ Bioreagents

H3K9me2/K14ac Antibody / Dimethyl-Histone H3 (Lys9) + Acetyl-Histone H3 (Lys14)

Product Code:
 
NSJ-R20346
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
RM322
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store the recombinant H3K9me2/K14ac antibody at -20°C.
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The H3K9me2/K14ac antibody specifically reacts to Histone H3 only when modified at both dimethyl-lysine 9 and acetyl-lysine 14 (K9me2/K14ac). No cross reactivity with non-modified Lysine 9/14, methylated Lysine 9 (K9me1, k9me2, k9me3) ONLY, acetylated Lysine 9/14 ONLY, or methylated Lysine 14 (K14me2) ONLY,
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The H3K9me2/K14ac antibody specifically reacts to Histone H3 only when modified at both dimethyl-lysine 9 and acetyl-lysine 14 (K9me2/K14ac). No cross reactivity with non-modified Lysine 9/14, methylated Lysine 9 (K9me1, k9me2, k9me3) ONLY, acetylated Lysine 9/14 ONLY, or methylated Lysine 14 (K14me2) ONLY,

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CodeSizePrice
NSJ-R20346-0.1ML100 ul£581.00
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Further Information

Application Details:
Western blot: 0.1ug/ml-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant H3K9me2/K14ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to Histone H3 only when modified by both methylation at lysine 9 and acetylation at lysine 14 (K9me2/K14ac). No cross reactivity with monomethylated Lysine 9 (K9me1), dimethylated Lysine 9 (K9me2), trimethylated Lysine 9 (K9me3), or acetylated Lysine 9/14 only, in histone H3.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A dimethyl-peptide corresponding to dimethyl-Histone H3 (Lys9) and an acetyl-peptide corresponding to Acetyl-Histone H3 (Lys14) was used as the immunogen for the H3K9me2/K14ac antibody.
Limitation:
This recombinant H3K9me2/K14ac antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P84243

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