NSJ Bioreagents

DNA-3-methyladenine glycosylase Antibody / MPG / AAG

Product Code:
 
NSJ-RQ7265
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
After reconstitution, the DNA-3-methyladenine glycosylase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Western blot testing of human 1) HepG2, 2) Jurkat and 3) 293T cell lysate with DNA-3-methyladenine glycosylase antibody. Predicted molecular weight ~33 kDa.

Western blot testing of human 1) HepG2, 2) Jurkat and 3) 293T cell lysate with DNA-3-methyladenine glycosylase antibody. Predicted molecular weight ~33 kDa.

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NSJ-RQ7265-100UG100 ug£535.00
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the DNA-3-methyladenine glycosylase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
MPG (N-methylpurine-DNA glycosylase) also known as MDG, 3-METHYLADENINE DNA GLYCOSYLASE, 3MeAde DNA GLYCOSYLASE, AAG and APNG is a protein expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line(HT29). The completely characterized human MPG gene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of Apng to the repair of several mutagenic lesions in vivo,Hang et al.(1997)biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the Apng gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine (meG) was found in liver sections of APNG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the APNG-null animals.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Q20-A298) was used as the immunogen for the DNA-3-methyladenine glycosylase antibody.
Limitation:
This DNA-3-methyladenine glycosylase antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P29372

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