ProSci

RGS8 Antibody

Product Code:
 
PSI-24-015
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Western Blot (WB)
Shipping:
 
Blue Ice
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 1
Western blot analysis of extracts of various cell lines, using RGS8 antibody (24-015) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Western blot analysis of extracts of various cell lines, using RGS8 antibody (24-015) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.

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CodeSizePrice
PSI-24-015-100uL100 uL£565.00
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Further Information

Application Note:
WB: 1:500 - 1:2000
Background:
This gene is a member of the regulator of G protein signaling (RGS) family and encodes a protein with a single RGS domain. Regulator of G protein signaling (RGS) proteins are regulatory and structural components of G protein-coupled receptor complexes. They accelerate transit through the cycle of GTP binding and hydrolysis to GDP, thereby terminating signal transduction, but paradoxically, also accelerate receptor-stimulated activation.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human RGS8 (NP_001095920.1).
NCBI Gene ID #:
85397
NCBI Official Name:
regulator of G-protein signaling 8
NCBI Official Symbol:
RGS8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Positive Control 1:
SH-SY5Y
Positive Control 2:
Jurkat
Positive Control 3:
Mouse brain
Positive Control 4:
Rat brain
Predicted Molecular Weight:
Observed: 17kDa/20kDa
Purification:
Affinity purification
Swissprot #:
P57771
User Note:
Optimal dilutions for each application to be determined by the researcher.