ProSci

PABPC5 Antibody

Product Code:
 
PSI-24-022
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Blue Ice
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 1
Western blot analysis of extracts of various cell lines, using PABPC5 antibody (24-022) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Western blot analysis of extracts of various cell lines, using PABPC5 antibody (24-022) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.

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PSI-24-022-100uL100 uL£565.00
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Further Information

Application Note:
WB: 1:1000 - 1:4000
IHC: 1:50 - 1:200
Background:
This gene encodes a protein that binds to the polyA tail found at the 3' end of most eukaryotic mRNAs. It is thought to play a role in the regulation of mRNA metabolic processes in the cytoplasm. This gene is located in a gene-poor region within the X-specific 13d-sY43 subinterval of the chromosome Xq21.3/Yp11.2 homology block. It is located close to translocation breakpoints associated with premature ovarian failure, and is therefore a potential candidate gene for this disorder.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-382 of human PABPC5 (NP_543022.1).
NCBI Gene ID #:
140886
NCBI Official Name:
poly(A) binding protein cytoplasmic 5
NCBI Official Symbol:
PABPC5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Positive Control 1:
SH-SY5Y
Positive Control 2:
A375
Positive Control 3:
MCF7
Positive Control 4:
Mouse liver
Positive Control 5:
Mouse skeletal muscle
Predicted Molecular Weight:
Observed: 43kDa
Purification:
Affinity purification
Swissprot #:
Q96DU9
User Note:
Optimal dilutions for each application to be determined by the researcher.