SARS-COV-2 Nucleocapsid Sandwich Elisa:
ELISA Capture: Recommended Concentration:0.5-2ug/mL, Sample: SARS-CoV-2 Nucleocapsid Protein Antibody?CAP)
ELISA Capture: Recommended Concentration:0.005-0.02ug/mL, Sample: SARS-CoV-2 Nucleocapsid Protein Antibody
Standard:Recommended Concentration: 156-10000pg/mL, Sample: Recombinant SARS-COV-2 Nucleocapsid Protein with His tag
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Lyophilized from a 0.2 um filtered solution in PBS, pH7.4 with antibody at a final concentration of 1 mg/ml.
E.coli derived SARS-COV-2 Nucleoproteins Ser2-Ala419 (Gly335Ala) Accession #QHD43423.2
Affinity purification < 1.0 EU/ug of the protein by LAL method.
Optimal dilutions for each application to be determined by the researcher.