ProSci

SARS-COV-2 Nucleocapsid Protein Antibody [3E8A5]

Product Code:
 
PSI-24-036
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
3E8A5
Regulatory Status:
 
RUO
Target Species:
 
Virus
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
 
Room Temp
Storage:
 
2°C - 8°C for one month, -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles
1 / 3
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
2 / 3
No cross-reactivity in ELISA with MERS-CoV Nucleoprotein protein and SARS Coronavirus Nucleocapsid.
3 / 3
Cat. No. 24-037, captured on COOH chip can bind AB1 with an affinity constant of 1.20nM as determined in a LSPR assay
❮❯

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
No cross-reactivity in ELISA with MERS-CoV Nucleoprotein protein and SARS Coronavirus Nucleocapsid.
Cat. No. 24-037, captured on COOH chip can bind AB1 with an affinity constant of 1.20nM as determined in a LSPR assay

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CodeSizePrice
PSI-24-036-0.1mg0.1 mg£686.00
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Further Information

Application Note:
SARS-COV-2 Nucleocapsid Sandwich Elisa:

ELISA Capture: Recommended Concentration:0.5-2ug/mL, Sample: SARS-CoV-2 Nucleocapsid Protein Antibody?CAP)

ELISA Capture: Recommended Concentration:0.005-0.02ug/mL, Sample: SARS-CoV-2 Nucleocapsid Protein Antibody

Standard:Recommended Concentration: 7.8-500pg/mL, Sample: Recombinant SARS-COV-2 Nucleocapsid Protein with His tag

Background:
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Buffer:
Lyophilized from a 0.2 um filtered solution in PBS, pH7.4
Concentration:
1 mg/ml
Conjugate:
Unconjugated
Immunogen:
E.coli derived SARS-COV-2 Nucleoproteins Ser2-Ala419 (Gly335Ala) Accession #QHD43423.2
NCBI Gene ID #:
1489678
NCBI Official Name:
Nucleocapsid phosphoprotein
NCBI Official Symbol:
N
NCBI Organism:
SARS-CoV-2
Physical State:
Lyophilized
Protein Accession #:
QHD43423.2
Purification:
Affinity purification < 1.0 EU/ug of the protein by LAL method.
Swissprot #:
P0DTC9
User Note:
Optimal dilutions for each application to be determined by the researcher.