ProSci

TMPRSS2 Antibody [ARC2373]

Product Code:
 
PSI-24-034
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
ARC1439
Regulatory Status:
 
RUO
Target Species:
 
Virus
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Blue Ice
Storage:
 
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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<strong>Figure 1 Western Blot Validation in Human Cell Lines and Mouse and Rat Tissues</strong><br>
Loading: 25 ?g of lysates per lane. Antibodies: TMPRSS2 antibody, 24-034, at 1:1000 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.
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<strong>Figure 2 Immunohistochemistry Validation of TMPRSS2 in Human Esophageal Tissue</strong><br> 
Immunohistochemistry of paraffin-embedded human esophageal tissue using TMPRSS2 antibody, 24-034, at 1:100 dilution (40x lens).

<strong>Figure 1 Western Blot Validation in Human Cell Lines and Mouse and Rat Tissues</strong><br>
Loading: 25 ?g of lysates per lane. Antibodies: TMPRSS2 antibody, 24-034, at 1:1000 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.
<strong>Figure 2 Immunohistochemistry Validation of TMPRSS2 in Human Esophageal Tissue</strong><br> 
Immunohistochemistry of paraffin-embedded human esophageal tissue using TMPRSS2 antibody, 24-034, at 1:100 dilution (40x lens).

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PSI-24-034-100ul100 ul£686.00
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Further Information

Application Note:
WB: 1:500 - 1:2000, IHC: 1:50 - 1:200
Background:
This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH 7.3
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Synthetic Peptide
NCBI Gene ID #:
7113
NCBI Official Name:
Transmembrane serine protease 2
NCBI Official Symbol:
TMPRSS2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
O15393
Purification:
Affinity purification
Swissprot #:
O15393
User Note:
Optimal dilutions for each application to be determined by the researcher.