ProSci

IL-2 Antibody [4N2Q9]

Product Code:
 
PSI-11-089
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
4N2Q9
Regulatory Status:
 
RUO
Target Species:
 
Virus
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
 
Blue Ice
Storage:
 
This antibody can be stored at 2°C to -8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.
1 / 4
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
2 / 4
From the comparison of the standard curve, it is found that ProSci has high sensitivity.
3 / 4
No significant cross-reactivity or interference was observed.
4 / 4
Placed at 37°C for 3 days, the stability of the standard curve all conform to CV <10%.
❮❯

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
From the comparison of the standard curve, it is found that ProSci has high sensitivity.
No significant cross-reactivity or interference was observed.
Placed at 37°C for 3 days, the stability of the standard curve all conform to CV <10%.

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CodeSizePrice
PSI-11-089-0.1mg0.1 mg£765.00
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  • Further Information
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Further Information

Application Note:
Human IL-2 Sanwich ELISA Immunoassay:

ELISA Capture: Recommended Concentration: 2-8ug/mL, Sample: Human IL-2 Monoclonal Antibody, Rabbit MAb (CAP)

ELISA Detection: Recommended Concentration: 0.15-0.5ug/mL, Sample: Human IL-2 Monoclonal Antibody, Rabbit Mab (DET)

Standard: Recommended Concentration: 0.78-50pg/mL, Sample: Recombinant Human IL2 Protein

Background:
Interleukin-2 (IL-2) is a O-glycosylated, four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma delta T cells, B cells, dendritic cells, and eosinophils. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis. IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells.
Buffer:
Supplied as a 0.2um filtered solution in PBS, pH 7.4.
Concentration:
1mg/ml
Conjugate:
Unconjugated
Immunogen:
Recombinant Human IL2 Protein, amino acids Ala21-Thr153
NCBI Gene ID #:
3558
NCBI Official Name:
interleukin 2
NCBI Official Symbol:
IL2
NCBI Organism:
Homo sapiens (human)
Physical State:
Liquid
Protein GI Number:
45593462
Purification:
Affinity Purified, Endotoxin Level: < 1.0 EU/ug of the protein by LAL method.
Swissprot #:
P60568(GiveninAccessionNumber)
User Note:
Optimal dilutions for each application to be determined by the researcher.