ProSci

IL-6 Antibody [2M8M3]

Product Code:
 
PSI-11-094
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
2M8M3
Regulatory Status:
 
RUO
Target Species:
 
Virus
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
 
Blue Ice
Storage:
 
This antibody can be stored at 2°C to -8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.
1 / 3
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
2 / 3
This assay recognizes natural and recombinant human IL-6.<br>The factors listed below were prepared at 10 ng/mL in calibrator diluent and assayed for cross-reactivity. Preparations of the following factors at 10 ng/mL in a mid-range recombinant human IL-6 control were assayed for interference. No significant cross-reactivity or interference was observed.
3 / 3
According to the relevant quality standards, the antibody was sold and placed at 37 degrees for accelerated stability test. After 20 days of destruction, the antibody performance remained stable.
❮❯

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes natural and recombinant human IL-6.<br>The factors listed below were prepared at 10 ng/mL in calibrator diluent and assayed for cross-reactivity. Preparations of the following factors at 10 ng/mL in a mid-range recombinant human IL-6 control were assayed for interference. No significant cross-reactivity or interference was observed.
According to the relevant quality standards, the antibody was sold and placed at 37 degrees for accelerated stability test. After 20 days of destruction, the antibody performance remained stable.

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CodeSizePrice
PSI-11-094-0.1mg0.1 mg£765.00
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Further Information

Application Note:
Human IL-6 Sandwich Immunoassay:

ELISA Capture: Recommended Concentration: 1-4ug/mL, Sample: Human IL-6 Monoclonal Antibody, Rabbit MAb(CAP)

ELISA Detection: Recommended Concentration: 0.03-0.15ug/mL, Sample: Human IL-6 Monoclonal Antibody, Rabbit MAb(DET)

Standard: Recommended Concentration: 0.78-50pg/mL, Sample: Recombinant Human IL6 Protein

Background:
Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable).
Buffer:
Supplied as a 0.2um filtered solution in PBS, pH 7.4.
Concentration:
1mg/ml
Conjugate:
Unconjugated
Immunogen:
E. coli-derived human IL-6 protein, amino acids Pro29-Met212
NCBI Gene ID #:
3569
NCBI Official Name:
interleukin 6
NCBI Official Symbol:
IL6
NCBI Organism:
Homo sapiens (human)
Physical State:
Liquid
Protein GI Number:
74739144
Purification:
Affinity Purified, Endotoxin Level: < 1.0 EU/ug of the protein by LAL method.
Swissprot #:
P05231
User Note:
Optimal dilutions for each application to be determined by the researcher.