ProSci

SARS-COV-2 Nucleocapsid Protein Antibody (biotin) [1B1A9]

Product Code:
 
PSI-24-043
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
1B1A9
Regulatory Status:
 
RUO
Target Species:
 
Virus
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
 
Blue Ice
Storage:
 
This antibody can be stored at 2°C - 8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.Avoid repeated freeze-thaw cycles.
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This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
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No cross-reactivity in ELISA with MERS-CoV Nucleoprotein protein and SARS Coronavirus Nucleocapsid.
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Cat. No. 24-037, captured on COOH chip can bind AB1 with an affinity constant of 1.20nM as determined in a LSPR assay .
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This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
No cross-reactivity in ELISA with MERS-CoV Nucleoprotein protein and SARS Coronavirus Nucleocapsid.
Cat. No. 24-037, captured on COOH chip can bind AB1 with an affinity constant of 1.20nM as determined in a LSPR assay .

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CodeSizePrice
PSI-24-043-0.1mg0.1 mg£1,042.00
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  • Further Information
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Further Information

Application Note:
SARS-COV-2 Nucleocapsid Sandwich Elisa:

ELISA Capture: Recommended Concentration:1-4ug/mL, Sample: SARS-CoV-2 Nucleocapsid Protein Antibody?CAP)

ELISA Detection: Recommended Concentration:0.05-0.2ug/mL, Sample: Biotinylated SARS-CoV-2 Nucleocapsid Protein Antibody

Standard:Recommended Concentration: 1.56-100pg/mL, Sample: Recombinant SARS-COV-2 Nucleocapsid Protein with His tag

Background:
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Buffer:
Supplied as a 0.2um filtered solution in PBS, pH 7.4 containing SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody at a concentration of 1mg/mL.
Concentration:
1 mg/ml
Conjugate:
biotin
Immunogen:
Hek293 derived SARS-COV-2 Nucleocapsid Ser2-Ala419 Accession #YP_009724397.2
NCBI Gene ID #:
1489678
NCBI Official Name:
Nucleocapsid phosphoprotein
NCBI Official Symbol:
N
NCBI Organism:
SARS-CoV-2
Physical State:
Liquid
Protein Accession #:
YP_009724397.2
Purification:
Affinity purification < 1.0 EU/ug of the protein by LAL method.
Swissprot #:
P0DTC9
User Note:
Optimal dilutions for each application to be determined by the researcher.