NSJ Bioreagents

SF3b145 Antibody / SAP145 / Splicing factor 3B subunit 2

Product Code:
 
NSJ-RQ7608
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the SF3b145 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of human SiHa cells with SF3b145 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SF3b145 antibody.
2 / 2
Western blot testing of 1) human Jurkat, 2) human HeLa, 3) human 293T, 4) human K562, 5) rat brain, 6) rat RH35, 7) mouse NIH 3T3 cell lysate with SF3b145 antibody. Predicted molecular weight ~100 kDa but commonly observed at up to ~145 kDa.
❮❯

Flow cytometry testing of human SiHa cells with SF3b145 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SF3b145 antibody.
Western blot testing of 1) human Jurkat, 2) human HeLa, 3) human 293T, 4) human K562, 5) rat brain, 6) rat RH35, 7) mouse NIH 3T3 cell lysate with SF3b145 antibody. Predicted molecular weight ~100 kDa but commonly observed at up to ~145 kDa.

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NSJ-RQ7608-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Splicing factor 3B subunit 2 is a protein that in humans is encoded by the SF3B2 gene. This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids H23-E832) was used as the immunogen for the SF3b145 antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q13435