NSJ Bioreagents

RNase H2 subunit B Antibody / RNASEH2B

Product Code:
 
NSJ-RQ7615
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RNase H2 subunit B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human T-47D cells with RNase H2 subunit B antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human MOLT4 cell lysate with RNase H2 subunit B antibody. Predicted molecular weight ~35 kDa.
3 / 3
Flow cytometry testing of human HL60 cells with RNase H2 subunit B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RNase H2 subunit B antibody.
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Immunofluorescent staining of FFPE human T-47D cells with RNase H2 subunit B antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human MOLT4 cell lysate with RNase H2 subunit B antibody. Predicted molecular weight ~35 kDa.
Flow cytometry testing of human HL60 cells with RNase H2 subunit B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RNase H2 subunit B antibody.

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CodeSizePrice
NSJ-RQ7615-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Ribonuclease H2, subunit B is a protein that in humans is encoded by the RNASEH2B gene. RNase H2 is composed of a single catalytic subunit (A) and two non-catalytic subunits (B and C) and specifically degrades the RNA of RNA:DNA hybrids. The protein encoded by this gene is the non-catalytic B subunit of RNase H2, which is thought to play a role in DNA replication. Multiple transcript variants encoding different isoforms have been found for this gene. Defects in this gene are a cause of Aicardi-Goutieres syndrome type 2 (AGS2).
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids K39-E260) was used as the immunogen for the RNase H2 subunit B antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q5TBB1