NSJ Bioreagents

SF3a120 Antibody / Splicing factor 3A subunit 1 / SF3A1

Product Code:
 
NSJ-RQ7633
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the SF3a120 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Western blot testing of 1) rat lung, 2) rat brain, 3) rat thymus, 4) rat C6, 5) mouse lung, 6) mouse brain, 7) mouse thymus and 8) mouse Neuro-2a cell lysate with SF3a120 antibody. Predicted molecular weight ~89 kDa but commonly observed at ~120 kDa.
2 / 12
Western blot testing of human 1) HeLa, 2) Jurkat, 3) K562, 4) 293T, 5) U-251, 6) Daudi and 7) MOLT4 cell lysate with SF3a120 antibody. Predicted molecular weight ~89 kDa but commonly observed at ~120 kDa.
3 / 12
IHC staining of FFPE human ovarian serous cancer tissue with SF3a120 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 12
IHC staining of FFPE human larynx squamous cell carcinoma tissue with SF3a120 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 12
IHC staining of FFPE human lung adenocarcinoma tissue with SF3a120 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 12
IHC staining of FFPE human spleen tissue with SF3a120 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 12
IHC staining of FFPE human prostate adenocarcinoma tissue with SF3a120 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 12
IHC staining of FFPE human liver cancer tissue with SF3a120 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 12
IHC staining of FFPE rat kidney tissue with SF3a120 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
10 / 12
Immunofluorescent staining of FFPE human intestinal cancer tissue with SF3a120 antibody. HIER: steam section in pH8 EDTA buffer for 20 min.
11 / 12
Immunofluorescent staining of FFPE rat colon tissue with SF3a120 antibody. HIER: steam section in pH8 EDTA buffer for 20 min.
12 / 12
Immunofluorescent staining of FFPE human PC-3 cells with SF3a120 antibody (red) and Alpha Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
❮❯

Western blot testing of 1) rat lung, 2) rat brain, 3) rat thymus, 4) rat C6, 5) mouse lung, 6) mouse brain, 7) mouse thymus and 8) mouse Neuro-2a cell lysate with SF3a120 antibody. Predicted molecular weight ~89 kDa but commonly observed at ~120 kDa.
Western blot testing of human 1) HeLa, 2) Jurkat, 3) K562, 4) 293T, 5) U-251, 6) Daudi and 7) MOLT4 cell lysate with SF3a120 antibody. Predicted molecular weight ~89 kDa but commonly observed at ~120 kDa.
IHC staining of FFPE human ovarian serous cancer tissue with SF3a120 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human larynx squamous cell carcinoma tissue with SF3a120 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with SF3a120 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with SF3a120 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate adenocarcinoma tissue with SF3a120 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with SF3a120 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat kidney tissue with SF3a120 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human intestinal cancer tissue with SF3a120 antibody. HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE rat colon tissue with SF3a120 antibody. HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human PC-3 cells with SF3a120 antibody (red) and Alpha Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.

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CodeSizePrice
NSJ-RQ7633-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Splicing factor 3 subunit 1 is a protein that in humans is encoded by the SF3A1 gene. This gene encodes a subunit of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer is a component of the mature U2 small nuclear ribonucleoprotein particle (snRNP). U2 small nuclear ribonucleoproteins play a critical role in spliceosome assembly and pre-mRNA splicing.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids K20-Q556) was used as the immunogen for the SF3a120 antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q15459