NSJ Bioreagents

hDKIR Antibody / C3IP1 / KLHL12

Product Code:
 
NSJ-RQ7635
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
2G11D1
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the hDKIR antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
IHC staining of FFPE human thyroid cancer tissue with hDKIR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human liver cancer tissue with hDKIR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 3
Western blot testing of 1) human PC-3, 2) human 293T, 3) human HeLa, 4) human Jurkat, 5) rat brain, 6) rat PC-12, 7) mouse brain and 8) mouse RAW264.7 cell lysate with hDKIR antibody. Predicted molecular weight ~63 kDa.
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IHC staining of FFPE human thyroid cancer tissue with hDKIR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with hDKIR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human PC-3, 2) human 293T, 3) human HeLa, 4) human Jurkat, 5) rat brain, 6) rat PC-12, 7) mouse brain and 8) mouse RAW264.7 cell lysate with hDKIR antibody. Predicted molecular weight ~63 kDa.

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NSJ-RQ7635-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Kelch-like protein 12, also called CUL3-interacting protein 1 (C3IP1) and DKIR homolog (hDKIR), is a protein that in humans is encoded by the KLHL12 gene. This gene encodes a member of the KLHL (Kelch-like) family of proteins. This protein has been identified as an autoantigen in the autoimmune disease Sjogren's syndrome and as a potential biomarker in primary biliary cirrhosis. This protein may act as a substrate adaptor of the Cullin-3 ubiquitin ligase complex to promote substrate-specific ubiquitylation. Ubiquitylation by this complex has been shown to regulate the Wnt signaling pathway as well as COPII vesicle coat size. A pseudogene has been identified on chromosome 22. Alternative splicing results in multiple transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R27-I331) was used as the immunogen for the hDKIR antibody.
Localisation:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q53G59