NSJ Bioreagents

Sideroflexin-5 Antibody / SFXN5

Product Code:
 
NSJ-RQ7638
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Sideroflexin-5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 13
IHC staining of FFPE human liver cancer tissue with Sideroflexin-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 13
IHC staining of FFPE human renal cancer tissue with Sideroflexin-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 13
IHC staining of FFPE human thyroid cancer tissue with Sideroflexin-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 13
IHC staining of FFPE human breast cancer tissue with Sideroflexin-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 13
IHC staining of FFPE human ovarian serous cancer tissue with Sideroflexin-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 13
IHC staining of FFPE human lung adenocarcinoma tissue with Sideroflexin-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 13
IHC staining of FFPE mouse brain tissue with Sideroflexin-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 13
IHC staining of FFPE mouse cerebellum tissue with Sideroflexin-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 13
IHC staining of FFPE rat brain tissue with Sideroflexin-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
10 / 13
IHC staining of FFPE rat cerebellum tissue with Sideroflexin-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
11 / 13
Western blot testing of 1) rat brain and 2) mouse brain tissue lysate with Sideroflexin-5 antibody. Predicted molecular weight ~37 kDa.
12 / 13
Flow cytometry testing of human U937 cells with Sideroflexin-5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Sideroflexin-5 antibody.
13 / 13
Flow cytometry testing of human A549 cells with Sideroflexin-5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Sideroflexin-5 antibody.
❮❯

IHC staining of FFPE human liver cancer tissue with Sideroflexin-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal cancer tissue with Sideroflexin-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thyroid cancer tissue with Sideroflexin-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with Sideroflexin-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian serous cancer tissue with Sideroflexin-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with Sideroflexin-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with Sideroflexin-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse cerebellum tissue with Sideroflexin-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Sideroflexin-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat cerebellum tissue with Sideroflexin-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat brain and 2) mouse brain tissue lysate with Sideroflexin-5 antibody. Predicted molecular weight ~37 kDa.
Flow cytometry testing of human U937 cells with Sideroflexin-5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Sideroflexin-5 antibody.
Flow cytometry testing of human A549 cells with Sideroflexin-5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Sideroflexin-5 antibody.

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CodeSizePrice
NSJ-RQ7638-100ug100 ug£535.00
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Sideroflexin-5 has cation transmembrane transporter activity and citrate transmembrane transporter activity. It is broadly expressed in brain, liver, kidney and other tissues. Sideroflexin-5 is associated with Parkinson Disease. An important paralog of this gene is SFXN3/Sideroflexin-3.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q34-L340) was used as the immunogen for the Sideroflexin-5 antibody.
Localisation:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q8TD22