NSJ Bioreagents

IFI16 Antibody / Gamma-interferon-inducible protein 16

Product Code:
 
NSJ-RQ7639
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
2I3D7
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the IFI16 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human U-251 cells with IFI16 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) Daudi, 2) Raji, 3) MOLT4 and 4) U-87 MG cell lysate with IFI16 antibody. Expected molecular weight: 85-95 kDa (three isoforms).
3 / 3
Flow cytometry testing of human U-251 cells with IFI16 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IFI16 antibody.
❮❯

Immunofluorescent staining of FFPE human U-251 cells with IFI16 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) Daudi, 2) Raji, 3) MOLT4 and 4) U-87 MG cell lysate with IFI16 antibody. Expected molecular weight: 85-95 kDa (three isoforms).
Flow cytometry testing of human U-251 cells with IFI16 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IFI16 antibody.

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CodeSizePrice
NSJ-RQ7639-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Gamma-interferon-inducible protein Ifi-16 (Ifi-16) also known as interferon-inducible myeloid differentiation transcriptional activator is a protein that in humans is encoded by the IFI16 gene. This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids E183-K743) was used as the immunogen for the IFI16 antibody.
Localisation:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q16666