NSJ Bioreagents

Shugoshin 2 Antibody / SGO2 / SGOL2

Product Code:
 
NSJ-RQ7640
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Shugoshin 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 4
Immunofluorescent staining of FFPE human U-2 OS cells with Shugoshin 2 antibody (green) and Alpha Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Western blot testing of human 1) HEL, 2) PC-3 and 3) MOLT4 cell lysate with Shugoshin 2 antibody. Predicted molecular weight ~145 kDa, commonly observed at 145-190 kDa.
3 / 4
Flow cytometry testing of human Caco-2 cells with Shugoshin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Shugoshin 2 antibody.
4 / 4
Flow cytometry testing of human RT4 cells with Shugoshin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Shugoshin 2 antibody.
❮❯

Immunofluorescent staining of FFPE human U-2 OS cells with Shugoshin 2 antibody (green) and Alpha Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HEL, 2) PC-3 and 3) MOLT4 cell lysate with Shugoshin 2 antibody. Predicted molecular weight ~145 kDa, commonly observed at 145-190 kDa.
Flow cytometry testing of human Caco-2 cells with Shugoshin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Shugoshin 2 antibody.
Flow cytometry testing of human RT4 cells with Shugoshin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Shugoshin 2 antibody.

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CodeSizePrice
NSJ-RQ7640-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Shugoshin 2 (SGO2), also called Shugoshin-like 2 (SGOL2), is predicted to be involved in homologous chromosome segregation; meiotic sister chromatid cohesion; and mitotic sister chromatid segregation. Predicted to act upstream of or within meiotic nuclear division; positive regulation of maintenance of meiotic sister chromatid cohesion, centromeric; and protein localization. Located in chromosome, centromeric region and nuclear body. Part of mitotic cohesion complex.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids N660-E1011) was used as the immunogen for the Shugoshin 2 antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q562F6