NSJ Bioreagents

SH2D4A Antibody / PPP1R38

Product Code:
 
NSJ-RQ7645
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the SH2D4A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 12
IHC staining of FFPE human liver cancer tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 12
IHC staining of FFPE human lung adenocarcinoma tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 12
IHC staining of FFPE human larynx squamous cell carcinoma tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 12
IHC staining of FFPE human ovarian serous cancer tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 12
IHC staining of FFPE human prostate adenocarcinoma tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 12
IHC staining of FFPE human breast cancer tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 12
IHC staining of FFPE human renal cancer tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 12
IHC staining of FFPE human spleen tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 12
IHC staining of FFPE rat gastric tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
10 / 12
Immunofluorescent staining of FFPE human A549 cells with SH2D4A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
11 / 12
Western blot testing of 1) human 293T, 2) human HeLa and 3) rat PC-12 cell lysate with SH2D4A antibody. Predicted molecular weight: ~53 kDa.
12 / 12
Flow cytometry testing of human A549 cells with SH2D4A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SH2D4A antibody.
❮❯

IHC staining of FFPE human liver cancer tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human larynx squamous cell carcinoma tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian serous cancer tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate adenocarcinoma tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal cancer tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat gastric tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A549 cells with SH2D4A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human 293T, 2) human HeLa and 3) rat PC-12 cell lysate with SH2D4A antibody. Predicted molecular weight: ~53 kDa.
Flow cytometry testing of human A549 cells with SH2D4A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SH2D4A antibody.

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CodeSizePrice
NSJ-RQ7645-100ug100 ug£535.00
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This product comes from: United States.
Typical lead time: 10-14 working days.
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  • Further Information
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
SH2 domain-containing protein 4A, also called Protein phosphatase 1 regulatory subunit 38 (PPP1R38) is a protein that in humans is encoded by the SH2D4A gene. Using exon trapping and exon linking at chromosome 8p22 cloned SH2D4A, which they called SH2A. The deduced 454-amino acid protein contains a single SH2 domain. RT-PCR and Northern blot analysis detected ubiquitous expression of 3 SH2A transcripts, as well as aberrant expression in some cancer cell lines. Dai et al. (2002) concluded that SH2A is a docking protein that may be involved in signal transduction.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R42-E454) was used as the immunogen for the SH2D4A antibody.
Localisation:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q9H788