NSJ Bioreagents

RNF216 Antibody / TRIAD3

Product Code:
 
NSJ-RQ7650
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RNF216 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of human HepG2 cells with RNF216 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RNF216 antibody.
2 / 2
Western blot testing of 1) human U-2 OS, 2) human MCF7, 3) human K562, 4) rat testis, 5) rat heart, 6) mouse testis and 7) mouse heart tissue lysate with RNF216 antibody. Predicted molecular weight ~99, ~106 and ~57 kDa (three isoforms).
❮❯

Flow cytometry testing of human HepG2 cells with RNF216 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RNF216 antibody.
Western blot testing of 1) human U-2 OS, 2) human MCF7, 3) human K562, 4) rat testis, 5) rat heart, 6) mouse testis and 7) mouse heart tissue lysate with RNF216 antibody. Predicted molecular weight ~99, ~106 and ~57 kDa (three isoforms).

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CodeSizePrice
NSJ-RQ7650-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
E3 ubiquitin-protein ligase RNF216 is an enzyme that in humans is encoded by the RNF216 gene. This gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for this locus but the full-length natures of only some are known.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids K538-R813) was used as the immunogen for the RNF216 antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q9NWF9