NSJ Bioreagents

SHROOM2 Antibody / APXL

Product Code:
 
NSJ-RQ7652
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the SHROOM2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of human U-2 OS cells with SHROOM2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SHROOM2 antibody.
2 / 2
Western blot testing of human 1) Caco-2 and 2) MCF7 cell lysate with SHROOM2 antibody. Predicted molecular weight ~176 kDa.
❮❯

Flow cytometry testing of human U-2 OS cells with SHROOM2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SHROOM2 antibody.
Western blot testing of human 1) Caco-2 and 2) MCF7 cell lysate with SHROOM2 antibody. Predicted molecular weight ~176 kDa.

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CodeSizePrice
NSJ-RQ7652-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Shroom family member 2 is a protein that in humans is encoded by the SHROOM2 gene. This gene represents the human homolog of Xenopus laevis apical protein (APX) gene, which is implicated in amiloride-sensitive sodium channel activity. It is expressed in endothelial cells and facilitates the formation of a contractile network within endothelial cells. Depletion of this gene results in an increase in endothelial sprouting, migration, and angiogenesis. This gene is highly expressed in the retina, and is a strong candidate for ocular albinism type 1 syndrome. Alternatively spliced transcript variants have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R427-H1138) was used as the immunogen for the SHROOM2 antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q13796