NSJ Bioreagents

Lamin A/C Antibody

Product Code:
 
NSJ-RQ7653
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
5F3C12
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Lamin A/C antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 5
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with Lamin A/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human liver cancer tissue with Lamin A/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human bladder epithelial carcinoma tissue with Lamin A/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
IHC staining of FFPE human ovarian cancer tissue with Lamin A/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 5
Western blot testing of 1) human placenta, 2) human HeLa, 3) human A431, 4) rat lung, 5) mouse lung and 6) mouse small intestine tissue lysate with Lamin A/C antibody. Predicted molecular weight ~74 kDa (A) & 65 kDa (C).
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IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with Lamin A/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with Lamin A/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human bladder epithelial carcinoma tissue with Lamin A/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer tissue with Lamin A/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human placenta, 2) human HeLa, 3) human A431, 4) rat lung, 5) mouse lung and 6) mouse small intestine tissue lysate with Lamin A/C antibody. Predicted molecular weight ~74 kDa (A) & 65 kDa (C).

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NSJ-RQ7653-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Lamins are structural protein components of the nuclear lamina, a protein network underlying the inner nuclear membrane that determines nuclear shape and size. There are three types of lamins, A,B and C. The lamin A/C (LMNA) gene contains 12 exons. Alternative splicing within exon 10 gives rise to two different mRNAs that code for pre-lamin A and lamin C. Lamin A/C is mapped to 1q21.2-q21.3 and mutations in this gene cause a variety of human diseases including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, and Hutchinson-Gilford progeria syndrome. Lamin A/C deficiency is thus associated with both defective nuclear mechanics and impaired mechanically activated gene transcription.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Y481-Y646) was used as the immunogen for the Lamin A/C antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
P02545