NSJ Bioreagents

XRCC1 Antibody / X-ray repair cross-complementing protein 1

Product Code:
 
NSJ-RQ7658
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
10E10.
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the XRCC1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human A431 cells with XRCC1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) HeLa, 2) 293T, 3) K562, 4) Jurkat, 5) HEL and 6) A431 cell lysate with XRCC1 antibody. Rountinely observed molecular weight: 69~90 kDa.
3 / 3
Flow cytometry testing of human HeLa cells with XRCC1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= XRCC1 antibody.
❮❯

Immunofluorescent staining of FFPE human A431 cells with XRCC1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) 293T, 3) K562, 4) Jurkat, 5) HEL and 6) A431 cell lysate with XRCC1 antibody. Rountinely observed molecular weight: 69~90 kDa.
Flow cytometry testing of human HeLa cells with XRCC1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= XRCC1 antibody.

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NSJ-RQ7658-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
XRCC1 (X-RAY REPAIR, COMPLEMENTING DEFECTIVE, IN CHINESE HAMSTER, 1) is a DNA repair protein which complexes with DNA ligase III. The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. The XRCC1 gene is mapped to 19q13.31. The XRCC1 interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. XRCC1 is phosphorylated in vivo and in vitro by CK2, and CK2 phosphorylation of XRCC1 on ser518, thr519, and thr523 largely determines aprataxin binding to XRCC1 through its FHA domain.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids E538-A633) was used as the immunogen for the XRCC1 antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
P18887