NSJ Bioreagents

Thymidine Kinase 2 Antibody / TK2

Product Code:
 
NSJ-RQ7667
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Thymidine Kinase 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of human Jurkat cells with Thymidine Kinase 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Thymidine Kinase 2 antibody.
2 / 2
Western blot testing of human 1) SiHa, 2) U-87 MG and 3) A549 cell lysate with Thymidine Kinase 2 antibody. Predicted molecular weight: 20-35 kDa (multiple isoforms).
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Flow cytometry testing of human Jurkat cells with Thymidine Kinase 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Thymidine Kinase 2 antibody.
Western blot testing of human 1) SiHa, 2) U-87 MG and 3) A549 cell lysate with Thymidine Kinase 2 antibody. Predicted molecular weight: 20-35 kDa (multiple isoforms).

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CodeSizePrice
NSJ-RQ7667-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Thymidine kinase 2, mitochondrial is a protein that in humans is encoded by the TK2 gene. This gene encodes a deoxyribonucleoside kinase that specifically phosphorylates thymidine, deoxycytidine, and deoxyuridine. The encoded enzyme localizes to the mitochondria and is required for mitochondrial DNA synthesis. Mutations in this gene are associated with a myopathic form of mitochondrial DNA depletion syndrome. Alternate splicing results in multiple transcript variants encoding distinct isoforms, some of which lack transit peptide, so are not localized to mitochondria.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids V34-P265) was used as the immunogen for the Thymidine Kinase 2 antibody.
Purity:
Antigen affinity purified
Uniprot #:
O00142