NSJ Bioreagents

TRMT61A Antibody

Product Code:
 
NSJ-RQ7670
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the TRMT61A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 15
IHC staining of FFPE human lung adenocarcinoma tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 15
IHC staining of FFPE human larynx squamous cell carcinoma tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 15
IHC staining of FFPE human colon adenocarcinoma tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 15
IHC staining of FFPE human breast cancer tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 15
IHC staining of FFPE human ovarian serous cancer tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 15
IHC staining of FFPE human spleen tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 15
IHC staining of FFPE human thyroid cancer tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 15
IHC staining of FFPE mouse brain tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 15
IHC staining of FFPE rat colon tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
10 / 15
IHC staining of FFPE rat spleen tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
11 / 15
IHC staining of FFPE rat skin tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
12 / 15
Immunofluorescent staining of FFPE human ovarian cancer tissue with TRMT61A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
13 / 15
Western blot testing of human 1) PC-3, 2) Caco-2, 3) MCF7 and 4) HaCaT cell lysate with TRMT61A antibody. Predicted molecular weight ~31 kDa.
14 / 15
Flow cytometry testing of human HepG2 cells with TRMT61A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRMT61A antibody.
15 / 15
Flow cytometry testing of human PC-3 cells with TRMT61A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRMT61A antibody.
❮❯

IHC staining of FFPE human lung adenocarcinoma tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human larynx squamous cell carcinoma tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon adenocarcinoma tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian serous cancer tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thyroid cancer tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat colon tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat spleen tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat skin tissue with TRMT61A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human ovarian cancer tissue with TRMT61A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Western blot testing of human 1) PC-3, 2) Caco-2, 3) MCF7 and 4) HaCaT cell lysate with TRMT61A antibody. Predicted molecular weight ~31 kDa.
Flow cytometry testing of human HepG2 cells with TRMT61A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRMT61A antibody.
Flow cytometry testing of human PC-3 cells with TRMT61A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRMT61A antibody.

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CodeSizePrice
NSJ-RQ7670-100ug100 ug£535.00
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This product comes from: United States.
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  • Further Information
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Enables mRNA (adenine-N1-)-methyltransferase activity. Involved in mRNA methylation. Predicted to be located in nucleoplasm. Predicted to be part of tRNA (m1A) methyltransferase complex. Predicted to be active in nucleus.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-G289) was used as the immunogen for the TRMT61A antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q96FX7