NSJ Bioreagents

mSin3A Antibody / SIN3A

Product Code:
 
NSJ-RQ7675
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the mSin3A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 9
Immunofluorescent staining of FFPE human ovarian cancer tissue with mSin3A antibody (red) and DAPI (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 9
Immunofluorescent staining of FFPE human U-2 OS cells with mSin3A antibody (green) and Alpha Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 9
IHC staining of FFPE human prostate adenocarcinoma tissue with mSin3A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 9
IHC staining of FFPE human lung adenocarcinoma tissue with mSin3A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 9
IHC staining of FFPE human larynx squamous cell carcinoma tissue with mSin3A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 9
IHC staining of FFPE human breast cancer tissue with mSin3A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 9
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human 293T, 4) human K562, 5) human MCF7 and 6) rat PC-12 cell lysate with mSin3A antibody. Predicted molecular weight ~145 kDa.
8 / 9
Flow cytometry testing of human SiHa cells with mSin3A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= mSin3A antibody.
9 / 9
Flow cytometry testing of human 293T cells with mSin3A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= mSin3A antibody.
❮❯

Immunofluorescent staining of FFPE human ovarian cancer tissue with mSin3A antibody (red) and DAPI (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human U-2 OS cells with mSin3A antibody (green) and Alpha Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human prostate adenocarcinoma tissue with mSin3A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with mSin3A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human larynx squamous cell carcinoma tissue with mSin3A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with mSin3A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human 293T, 4) human K562, 5) human MCF7 and 6) rat PC-12 cell lysate with mSin3A antibody. Predicted molecular weight ~145 kDa.
Flow cytometry testing of human SiHa cells with mSin3A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= mSin3A antibody.
Flow cytometry testing of human 293T cells with mSin3A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= mSin3A antibody.

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CodeSizePrice
NSJ-RQ7675-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Paired amphipathic helix protein Sin3a is a protein that in humans is encoded by the SIN3A gene. The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q8-E918) was used as the immunogen for the mSin3A antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q96ST3