NSJ Bioreagents

SLC25A24 Antibody / Mit°Chondrial ATP-Mg/Pi carrier protein 1

Product Code:
 
NSJ-RQ7686
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the SLC25A24 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 14
Immunofluorescent staining of FFPE human colon cancer tissue with SLC25A24 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 14
IHC staining of FFPE human lung adenocarcinoma tissue with SLC25A24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 14
IHC staining of FFPE human testicular seminoma tissue with SLC25A24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 14
IHC staining of FFPE human diffuse large B cell lymphoma tissue with SLC25A24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 14
IHC staining of FFPE human duodenal papilla adenocarcinoma tissue with SLC25A24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 14
IHC staining of FFPE human endometrioid adenocarcinoma tissue with SLC25A24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 14
IHC staining of FFPE human glioblastoma tissue with SLC25A24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 14
IHC staining of FFPE human larynx squamous cell carcinoma tissue with SLC25A24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 14
IHC staining of FFPE human liver cancer tissue with SLC25A24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
10 / 14
IHC staining of FFPE human prostate adenocarcinoma tissue with SLC25A24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
11 / 14
IHC staining of FFPE human placental tissue with SLC25A24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
12 / 14
IHC staining of FFPE human spleen tissue with SLC25A24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
13 / 14
Western blot testing of human 1) 293T, 2) placenta, 3) A431 and 4) MCF7 cell lysate with SLC25A24 antibody. Predicted molecular weight: 51-53 kDa (two isoforms).
14 / 14
Flow cytometry testing of human MCF7 cells with SLC25A24 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SLC25A24 antibody.

Immunofluorescent staining of FFPE human colon cancer tissue with SLC25A24 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human lung adenocarcinoma tissue with SLC25A24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human testicular seminoma tissue with SLC25A24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human diffuse large B cell lymphoma tissue with SLC25A24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human duodenal papilla adenocarcinoma tissue with SLC25A24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human endometrioid adenocarcinoma tissue with SLC25A24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human glioblastoma tissue with SLC25A24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human larynx squamous cell carcinoma tissue with SLC25A24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with SLC25A24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate adenocarcinoma tissue with SLC25A24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with SLC25A24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with SLC25A24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) 293T, 2) placenta, 3) A431 and 4) MCF7 cell lysate with SLC25A24 antibody. Predicted molecular weight: 51-53 kDa (two isoforms).
Flow cytometry testing of human MCF7 cells with SLC25A24 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SLC25A24 antibody.

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NSJ-RQ7686-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Calcium-binding mitochondrial carrier protein SCaMC-1 is a protein that in humans is encoded by the SLC25A24 gene. This gene encodes a carrier protein that transports ATP-Mg exchanging it for phosphate. Multiple transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-I293) was used as the immunogen for the SLC25A24 antibody.
Localisation:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q6NUK1