NSJ Bioreagents

EIF2AK1 Antibody / Eukaryotic translation initiation factor 2-alpha kinase 1 / PKR

Product Code:
 
NSJ-RQ7687
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the EIF2AK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human A549 cells with EIF2AK1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) HeLa, 2) 293T, 3) MCF7, 4) K562, 5) PANC-1, 6) A431 and 7) U-251 cell lysate with EIF2AK1 antibody. Predicted molecular weight ~62 kDa but routinely observed at 68-72 kDa.
3 / 3
Flow cytometry testing of human HEL cells with EIF2AK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EIF2AK1 antibody.
❮❯

Immunofluorescent staining of FFPE human A549 cells with EIF2AK1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) 293T, 3) MCF7, 4) K562, 5) PANC-1, 6) A431 and 7) U-251 cell lysate with EIF2AK1 antibody. Predicted molecular weight ~62 kDa but routinely observed at 68-72 kDa.
Flow cytometry testing of human HEL cells with EIF2AK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EIF2AK1 antibody.

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CodeSizePrice
NSJ-RQ7687-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Eukaryotic translation initiation factor 2-alpha kinase 1 is an enzyme that in humans is encoded by the EIF2AK1 gene. The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids E37-D508) was used as the immunogen for the EIF2AK1 antibody.
Localisation:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q9BQI3