NSJ Bioreagents

PSMB7 Antibody / Proteasome 20S beta 7

Product Code:
 
NSJ-RQ7689
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
3H6C8
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PSMB7 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of human Caco-2 cells with PSMB7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PSMB7 antibody.
2 / 2
Western blot testing of 1) human HepG2, 2) human HeLa, 3) human PC-3, 4) monkey COS-7, 5) rat brain, 6) rat liver, 7) mouse brain and 8) mouse liver tissue lysate with PSMB7 antibody. Predicted molecular weight ~30 kDa.
❮❯

Flow cytometry testing of human Caco-2 cells with PSMB7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PSMB7 antibody.
Western blot testing of 1) human HepG2, 2) human HeLa, 3) human PC-3, 4) monkey COS-7, 5) rat brain, 6) rat liver, 7) mouse brain and 8) mouse liver tissue lysate with PSMB7 antibody. Predicted molecular weight ~30 kDa.

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NSJ-RQ7689-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Proteasome subunit beta type-7 as known as 20S proteasome subunit beta-2 is a protein that in humans is encoded by the PSMB7 gene. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. A pseudogene of this gene is located on the long arm of chromosome 14.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D17-S277) was used as the immunogen for the PSMB7 antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q99436