NSJ Bioreagents

Citrin Antibody / ARALAR2 / SLC25A13

Product Code:
 
NSJ-RQ7700
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Citrin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 15
Immunofluorescent staining of FFPE human liver cancer tissue with Citrin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 15
Immunofluorescent staining of FFPE human colon cancer tissue with Citrin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
3 / 15
Immunofluorescent staining of FFPE human A549 cells with Citrin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 15
IHC staining of FFPE human liver cancer tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 15
IHC staining of FFPE human brain tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 15
IHC staining of FFPE human diffuse large B cell lymphoma tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 15
IHC staining of FFPE human colon adenocarcinoma tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 15
IHC staining of FFPE human breast cancer tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 15
IHC staining of FFPE human appendix adenocarcinoma tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
10 / 15
IHC staining of FFPE human right renal oncocytoma tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
11 / 15
IHC staining of FFPE human prostate adenocarcinoma tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
12 / 15
IHC staining of FFPE human urothelial carcinoma tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
13 / 15
Western blot testing of 1) human Raji, 2) human A431, 3) human 293T, 4) human HepG2, 5) rat kidney and 6) rat liver tissue lysate with Citrin antibody. Predicted molecular weight ~74 kDa.
14 / 15
Flow cytometry testing of human JK-1 cells with Citrin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Citrin antibody.
15 / 15
Flow cytometry testing of human U-87 MG cells with Citrin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Citrin antibody.
❮❯

Immunofluorescent staining of FFPE human liver cancer tissue with Citrin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human colon cancer tissue with Citrin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human A549 cells with Citrin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human liver cancer tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human brain tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human diffuse large B cell lymphoma tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon adenocarcinoma tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human appendix adenocarcinoma tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human right renal oncocytoma tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate adenocarcinoma tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human urothelial carcinoma tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human Raji, 2) human A431, 3) human 293T, 4) human HepG2, 5) rat kidney and 6) rat liver tissue lysate with Citrin antibody. Predicted molecular weight ~74 kDa.
Flow cytometry testing of human JK-1 cells with Citrin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Citrin antibody.
Flow cytometry testing of human U-87 MG cells with Citrin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Citrin antibody.

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CodeSizePrice
NSJ-RQ7700-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Citrin also known as solute carrier family 25, member 13 (also called Citrin and ARALAR-related gene 2) is a protein which in humans is encoded by the SLC25A13 gene. This gene is a member of the mitochondrial carrier family. The encoded protein contains four EF-hand Ca(2+) binding motifs in the N-terminal domain, and localizes to mitochondria. The protein catalyzes the exchange of aspartate for glutamate and a proton across the inner mitochondrial membrane, and is stimulated by calcium on the external side of the inner mitochondrial membrane. Mutations in this gene result in citrullinemia, type II. Multiple transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-A643) was used as the immunogen for the Citrin antibody.
Localisation:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q9UJS0