NSJ Bioreagents

Thiosulfate sulfurtransferase Antibody / TST

Product Code:
 
NSJ-RQ7702
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Thiosulfate sulfurtransferase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 13
Immunofluorescent staining of FFPE human lung cancer tissue with Thiosulfate sulfurtransferase antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 13
Immunofluorescent staining of FFPE human A549 cells with Thiosulfate sulfurtransferase antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 13
IHC staining of FFPE human right renal oncocytoma tissue with Thiosulfate sulfurtransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 13
IHC staining of FFPE human spleen tissue with Thiosulfate sulfurtransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 13
IHC staining of FFPE human prostate adenocarcinoma tissue with Thiosulfate sulfurtransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 13
IHC staining of FFPE human lung adenocarcinoma tissue with Thiosulfate sulfurtransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 13
IHC staining of FFPE human diffuse large B-cell lymphoma tissue with Thiosulfate sulfurtransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 13
IHC staining of FFPE human appendix adenocarcinoma tissue with Thiosulfate sulfurtransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 13
IHC staining of FFPE human colon adenocarcinoma tissue with Thiosulfate sulfurtransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
10 / 13
IHC staining of FFPE human endometrioid adenocarcinoma tissue with Thiosulfate sulfurtransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
11 / 13
IHC staining of FFPE human placental tissue with Thiosulfate sulfurtransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
12 / 13
IHC staining of FFPE human glioblastoma tissue with Thiosulfate sulfurtransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
13 / 13
Western blot testing of 1) human 293T, 2) human HepG2, 3) human MCF7, 4) rat kidney, 5) rat liver and 6) mouse liver tissue lysate with Thiosulfate sulfurtransferase antibody. Predicted molecular weight ~33 kDa but may be observed at higher molecular weights due to glycosylation.
❮❯

Immunofluorescent staining of FFPE human lung cancer tissue with Thiosulfate sulfurtransferase antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human A549 cells with Thiosulfate sulfurtransferase antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human right renal oncocytoma tissue with Thiosulfate sulfurtransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with Thiosulfate sulfurtransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate adenocarcinoma tissue with Thiosulfate sulfurtransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with Thiosulfate sulfurtransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human diffuse large B-cell lymphoma tissue with Thiosulfate sulfurtransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human appendix adenocarcinoma tissue with Thiosulfate sulfurtransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon adenocarcinoma tissue with Thiosulfate sulfurtransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human endometrioid adenocarcinoma tissue with Thiosulfate sulfurtransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with Thiosulfate sulfurtransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human glioblastoma tissue with Thiosulfate sulfurtransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human 293T, 2) human HepG2, 3) human MCF7, 4) rat kidney, 5) rat liver and 6) mouse liver tissue lysate with Thiosulfate sulfurtransferase antibody. Predicted molecular weight ~33 kDa but may be observed at higher molecular weights due to glycosylation.

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NSJ-RQ7702-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
This is one of two neighboring genes encoding similar proteins that each contain two rhodanese domains. The encoded protein is localized to the mitochondria and catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. In addition, the protein interacts with 5S ribosomal RNA and facilitates its import into the mitochondria. Alternative splicing results in multiple transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-D220) was used as the immunogen for the Thiosulfate sulfurtransferase antibody.
Localisation:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q16762