NSJ Bioreagents

EHD4 Antibody / EH domain-containing protein 4

Product Code:
 
NSJ-RQ7717
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the EHD4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 4
Western blot testing of 1) rat pancreas, 2) rat heart, 3) rat testis, 4) rat C6, 5) mouse pancreas, 6) mouse heart, 7) mouse testis and 8) mouse RAW264.7 cell lysate with EHD4 antibody. Predicted molecular weight ~61 kDa.
2 / 4
Western blot testing of 1) human HeLa, 2) human HepG2, 3) monkey heart, 4) human placenta, 5) human Caco-2 and 6) human PC-3 cell lysate with EHD4 antibody. Predicted molecular weight ~61 kDa.
3 / 4
Flow cytometry testing of human U-2 OS cells with EHD4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EHD4 antibody.
4 / 4
Flow cytometry testing of human U-251 cells with EHD4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EHD4 antibody.
❮❯

Western blot testing of 1) rat pancreas, 2) rat heart, 3) rat testis, 4) rat C6, 5) mouse pancreas, 6) mouse heart, 7) mouse testis and 8) mouse RAW264.7 cell lysate with EHD4 antibody. Predicted molecular weight ~61 kDa.
Western blot testing of 1) human HeLa, 2) human HepG2, 3) monkey heart, 4) human placenta, 5) human Caco-2 and 6) human PC-3 cell lysate with EHD4 antibody. Predicted molecular weight ~61 kDa.
Flow cytometry testing of human U-2 OS cells with EHD4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EHD4 antibody.
Flow cytometry testing of human U-251 cells with EHD4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EHD4 antibody.

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CodeSizePrice
NSJ-RQ7717-100ug100 ug£535.00
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
EH-domain containing 4, also known as EHD4, is a human gene belonging to the EHD protein family. Enables cadherin binding activity. Involved in endocytic recycling and protein homooligomerization. Located in endoplasmic reticulum and recycling endosome membrane.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R12-D541) was used as the immunogen for the EHD4 antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q9H223