NSJ Bioreagents

CHGA Antibody / Chromogranin A

Product Code:
 
NSJ-RQ7726
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the CHGA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 5
IHC staining of FFPE human lung adenocarcinoma tissue with CHGA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human testicular germ cell tumor tissue with CHGA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human colorectal adenocarcinoma tissue with CHGA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human SH-SY5Y cell lysate with CHGA antibody. Predicted molecular weight ~50 kDa but may be observed at higher molecular weights due to glycosylation.
5 / 5
Flow cytometry testing of human MCF7 cells with CHGA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CHGA antibody.
❮❯

IHC staining of FFPE human lung adenocarcinoma tissue with CHGA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human testicular germ cell tumor tissue with CHGA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with CHGA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human SH-SY5Y cell lysate with CHGA antibody. Predicted molecular weight ~50 kDa but may be observed at higher molecular weights due to glycosylation.
Flow cytometry testing of human MCF7 cells with CHGA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CHGA antibody.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-RQ7726-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Show All

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
CHGA, also known as CGA or Chromogranin A, is an acidic protein co-stored and co-released with catecholamines from storage granules in the adrenal medulla. CHGA is a member of the granin family of neuroendocrine secretory protein, It is located in secretory vesicles of neurons and endocrine cells. In humans, CHGA protein is encoded by the CHGA gene, and it is mapped to 14q32.12. CHGA gene product is a precursor to three biologically active peptides: vasostatin, pancreastatin, and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine).
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids L19-E417) was used as the immunogen for the CHGA antibody.
Localisation:
Finely granular cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P10645