NSJ Bioreagents

DBL Antibody / MCF2

Product Code:
 
NSJ-RQ7780
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the DBL antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Flow cytometry testing of human MCF7 cells with DBL antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DBL antibody.
2 / 3
Flow cytometry testing of human U-251 cells with DBL antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DBL antibody.
3 / 3
Western blot testing of human SH-SY5Y cell lysate with DBL antibody. Predicted molecular weight: 96-116 kDa (multiple isoforms).
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Flow cytometry testing of human MCF7 cells with DBL antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DBL antibody.
Flow cytometry testing of human U-251 cells with DBL antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DBL antibody.
Western blot testing of human SH-SY5Y cell lysate with DBL antibody. Predicted molecular weight: 96-116 kDa (multiple isoforms).

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CodeSizePrice
NSJ-RQ7780-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
The DBL proto-oncogene is a protein that in humans is encoded by the MCF2 gene. The oncogenic protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that exerts control over some members of the Rho family of small GTPases. Several transcript variants encoding different isoforms have been found for this gene. These isoforms exhibit different expression patterns and varying levels of GEF activity.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D42-Y925) was used as the immunogen for the DBL antibody.
Purity:
Antigen affinity purified
Uniprot #:
P10911