NSJ Bioreagents

UBXN8 Antibody / UBX domain-containing protein 8

Product Code:
 
NSJ-RQ7781
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the UBXN8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 4
IHC staining of FFPE human testicular seminoma tissue with UBXN8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
Western blot testing of human 1) HEL, 2) K562 and 3) HepG2 cell lysate with UBXN8 antibody. Predicted molecular weight ~31 kDa.
3 / 4
Flow cytometry testing of human RT4 cells with UBXN8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= UBXN8 antibody.
4 / 4
Flow cytometry testing of human U937 cells with UBXN8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= UBXN8 antibody.
❮❯

IHC staining of FFPE human testicular seminoma tissue with UBXN8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HEL, 2) K562 and 3) HepG2 cell lysate with UBXN8 antibody. Predicted molecular weight ~31 kDa.
Flow cytometry testing of human RT4 cells with UBXN8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= UBXN8 antibody.
Flow cytometry testing of human U937 cells with UBXN8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= UBXN8 antibody.

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CodeSizePrice
NSJ-RQ7781-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
p97 or VCP (valosin-containing protein) is a versatile ATPase complex, and many cofactors are required for the p97 functional diversity. This gene encodes one of the p97 cofactors. This cofactor is a transmembrane protein and localized in the endoplasmic reticulum (ER) membrane. It tethers p97 to the ER membrane via its UBX domain. The association of this cofactor with p97 facilitates efficient ER-associated degradation of misfolded proteins. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids N149-N270) was used as the immunogen for the UBXN8 antibody.
Localisation:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
O00124