NSJ Bioreagents

TTLL12 Antibody / Tubulin Tyrosine Ligase Like 12

Product Code:
 
NSJ-RQ7797
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the TTLL12 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 5
IHC staining of FFPE human colorectal adenocarcinoma tissue with TTLL12 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human thyroid cancer tissue with TTLL12 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
Western blot testing of human 1) K562 and 2) 293T cell lysate with TTLL12 antibody. Predicted molecular weight ~74 kDa.
4 / 5
Flow cytometry testing of human HEL cells with TTLL12 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TTLL12 antibody.
5 / 5
Flow cytometry testing of human Caco-2 cells with TTLL12 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TTLL12 antibody.
❮❯

IHC staining of FFPE human colorectal adenocarcinoma tissue with TTLL12 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thyroid cancer tissue with TTLL12 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) K562 and 2) 293T cell lysate with TTLL12 antibody. Predicted molecular weight ~74 kDa.
Flow cytometry testing of human HEL cells with TTLL12 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TTLL12 antibody.
Flow cytometry testing of human Caco-2 cells with TTLL12 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TTLL12 antibody.

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CodeSizePrice
NSJ-RQ7797-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Immunohistochemistry (FFPE): 2-5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Enables H4K20me3 modified histone binding activity and tubulin binding activity. Involved in negative regulation of type I interferon-mediated signaling pathway and regulation of mitotic cell cycle. Located in cytoplasm.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids E8-Q634) was used as the immunogen for the TTLL12 antibody.
Localisation:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q14166