NSJ Bioreagents

B7-H3 Antibody / CD276

Product Code:
 
NSJ-RQ7825
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the B7-H3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 5
IHC staining of FFPE human tonsil tissue with B7-H3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human liver cancer tissue with B7-H3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human rectal cancer tissue with B7-H3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human 1) U-2 OS, 2) PC-3, 3) HeLa and 4) MCF7 cell lysate with B7-H3 antibody. Expected molecular weight: 57-110 kDa depending on level of glycosylation.
5 / 5
Flow cytometry testing of human PC-3 cells with B7-H3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= B7-H3 antibody.
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IHC staining of FFPE human tonsil tissue with B7-H3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with B7-H3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer tissue with B7-H3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) U-2 OS, 2) PC-3, 3) HeLa and 4) MCF7 cell lysate with B7-H3 antibody. Expected molecular weight: 57-110 kDa depending on level of glycosylation.
Flow cytometry testing of human PC-3 cells with B7-H3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= B7-H3 antibody.

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CodeSizePrice
NSJ-RQ7825-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Cluster of Differentiation 276 (CD276) or B7 Homolog 3 (B7-H3) is a human protein encoded by the CD276 gene. The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids L29-Q458) was used as the immunogen for the B7-H3 antibody.
Localisation:
Cytoplasm, Cell surface
Purity:
Antigen affinity purified
Uniprot #:
Q5ZPR3