NSJ Bioreagents

RREB1 Antibody / Ras-responsive element-binding protein 1

Product Code:
 
NSJ-RQ7917
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RREB1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of human HeLa cells with RREB1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RREB1 antibody.
2 / 2
Western blot testing of human 1) HeLa, 2) PC-3 and 3) SH-SY5Y cell lysate with RREB1 antibody. Predicted molecular weight: 153-188 (multiple isoforms) with two smaller isoforms.
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Flow cytometry testing of human HeLa cells with RREB1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RREB1 antibody.
Western blot testing of human 1) HeLa, 2) PC-3 and 3) SH-SY5Y cell lysate with RREB1 antibody. Predicted molecular weight: 153-188 (multiple isoforms) with two smaller isoforms.

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CodeSizePrice
NSJ-RQ7917-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Ras-responsive element-binding protein 1 is a protein that in humans is encoded by the RREB1 gene. The protein encoded by this gene is a zinc finger transcription factor that binds to RAS-responsive elements (RREs) of gene promoters. It has been shown that the calcitonin gene promoter contains an RRE and that the encoded protein binds there and increases expression of calcitonin, which may be involved in Ras/Raf-mediated cell differentiation. Multiple transcript variants encoding several different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids K732-Q1559) was used as the immunogen for the RREB1 antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q92766