NSJ Bioreagents

PDGFR alpha Antibody / PDGFRA / PDGFR2

Product Code:
 
NSJ-RQ7956
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PDGFR alpha antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Immunofluorescent staining of FFPE human Caco-2 cells with PDGFR alpha antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 2
Western blot testing of 1) human 293T, 2) human U-251, 3) rat brain, 4) rat C6, 5) mouse brain and 6) mouse NIH 3T3 cell lysate with PDGFR alpha antibody. Expected molecular weight: 120-195 kDa depending on glycosylation level.
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Immunofluorescent staining of FFPE human Caco-2 cells with PDGFR alpha antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human 293T, 2) human U-251, 3) rat brain, 4) rat C6, 5) mouse brain and 6) mouse NIH 3T3 cell lysate with PDGFR alpha antibody. Expected molecular weight: 120-195 kDa depending on glycosylation level.

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NSJ-RQ7956-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
PDGFRA (Platelet-derived growth factor receptor, alpha), also called PDGFR2, encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. The PDGFA gene is mapped on 4q12. The PDGFRA-FIP1L1 gene is a constitutively activated tyrosine kinase that transforms hematopoietic cells and is a therapeutic target of imatinib. The PDGFRA gene contains 23 exons spanning about 65 kb. Using the human PDGFRA promoter linked to a luciferase reporter, Joosten et al. showed that PAX1 acts as a transcriptional activator of the PDGFRA gene in differentiated human embryonal carcinoma cells. PDGFRA is responsible for mediating cellular contraction of multiple growth factors: TGFB1 and members of the PDGF family. Lei et al. noted that in the rabbit model of the disease, PDGFRA is dramatically more capable of promoting PVR than is the closely related PDGFRB. PDGFRA is a critical receptor required for human CMV infection, and thus a target for novel antiviral therapies.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q24-E906) was used as the immunogen for the PDGFR alpha antibody.
Localisation:
Cytoplasm, cell membrane
Purity:
Antigen affinity purified
Uniprot #:
P16234