NSJ Bioreagents

RPRD1B Antibody / C20orf77

Product Code:
 
NSJ-RQ7960
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Monkey
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RPRD1B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Immunofluorescent staining of FFPE human U-2 OS cells with RPRD1B antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 8
Immunofluorescent staining of FFPE human esophageal squamous cell carcinoma tissue with RPRD1B antibody (red). HIER: steam section in pH8 EDTA buffer for 20 min.
3 / 8
IHC staining of FFPE human B cell lymphoma tissue with RPRD1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE human esophageal squamous carcinoma tissue with RPRD1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE human esophageal squamous carcinoma tissue with RPRD1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 8
IHC staining of FFPE human rectal cancer tissue with RPRD1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 8
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human Caco-2 and 4) monkey COS-7 cell lysate with RPRD1B antibody. Predicted molecular weight ~37 kDa.
8 / 8
Flow cytometry testing of human HeLa cells with RPRD1B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RPRD1B antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with RPRD1B antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human esophageal squamous cell carcinoma tissue with RPRD1B antibody (red). HIER: steam section in pH8 EDTA buffer for 20 min.
IHC staining of FFPE human B cell lymphoma tissue with RPRD1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous carcinoma tissue with RPRD1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous carcinoma tissue with RPRD1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer tissue with RPRD1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human Caco-2 and 4) monkey COS-7 cell lysate with RPRD1B antibody. Predicted molecular weight ~37 kDa.
Flow cytometry testing of human HeLa cells with RPRD1B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RPRD1B antibody.

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CodeSizePrice
NSJ-RQ7960-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Enables RNA polymerase II complex binding activity and identical protein binding activity. Involved in positive regulation of cell population proliferation; regulation of cell cycle process; and regulation of transcription by RNA polymerase II. Located in nucleoplasm. Part of RNA polymerase II, holoenzyme.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R79-K272) was used as the immunogen for the RPRD1B antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q9NQG5